Role of lipid raft in assembly of human herpesvirus 6.
- Author:
Hong-lan HUANG
1
;
Fan LI
Author Information
1. Dept. of Pathogenobiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China.
- Publication Type:Journal Article
- MeSH:
CD59 Antigens;
analysis;
Fluorescent Antibody Technique;
G(M1) Ganglioside;
analysis;
Herpesvirus 6, Human;
physiology;
Membrane Microdomains;
physiology;
Viral Envelope Proteins;
analysis;
Virus Assembly
- From:
Chinese Journal of Virology
2008;24(4):295-299
- CountryChina
- Language:Chinese
-
Abstract:
To explore the role of lipid raft in assembly of human herpesvirus 6, the HHV-6 GS strain was applied to infect the HSB2 cells and then the lipid raft composition was extracted from the cells with non-ionic detergent Triton-X 100. The relationship between the HHV-6 envelope glycoprotein and lipid raft was analyzed by Western Blot. Immunofluorescence double-staining was used to study the colocalization of the HHV-6 glycoprotein B(gB) with GPI anchored protein CD59 and ganglioside GM respectively. HHV-6 envelope glycoprotein B, H, L, Q1 and Q2 (gB, gH, gL, gQ1 and gQ2) were all existed in the lipid raft. Moreover, CD59 and HHV-6 envelope glycoprotein B showed the same localization through the confocal microscope. We concluded the lipid raft provided the platform for HHV-6 assembly. This is the first report concerning to the role of lipid raft in assembly of human Herpesvirus 6.
