Immune response induced by human cytomegalovirus DNA vaccine.
- Author:
Meng LI
1
;
Li-jun SHAO
;
Li-jun GUO
;
Xuan-jun WANG
Author Information
1. Department of Clinical Medicine, Weifang Medical College, Weifang 261042, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Viral;
blood;
COS Cells;
Cercopithecus aethiops;
Cytomegalovirus Vaccines;
immunology;
Female;
Humans;
Mice;
Mice, Inbred BALB C;
Vaccines, DNA;
immunology
- From:
Chinese Journal of Virology
2008;24(4):320-321
- CountryChina
- Language:Chinese
-
Abstract:
Human cytomegalovirus is an important pathogen which widely infects human. In this study, an eukaryotic expression vector containing human cytomegalovirus gB680 and pp65m genes was injected into BALB/c mice to explore the immune response. Firstly, the recombinant plasmid pVAX1/gB680+pp65m and plasmid pVAX1 were transfected into COS-7 cells respectively and the transiently expressed product was detected by RT-PCR and Western blot. The maxiprepared plasmid pVAX1/gB680 + pp65m and pVAX1 by alkaline lysis with SDS and purified by Sepharose 4FF column chromatography were then used to immunize BALB/c mice and the humoral and cellular immune responses were determined. Recombinant plasmid pVAX1/gB680+pp65m could be expressed in COS-7 cells, and could induce antibodies and cellular immune responses in BALB/c mice.
