The identification and sequence analysis of ALV-J isolated from layers.
- Author:
Hui WANG
1
;
Zhi-Zhong CUI
Author Information
1. College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018.
- Publication Type:Journal Article
- MeSH:
Animals;
Avian Leukosis Virus;
classification;
isolation & purification;
Base Sequence;
Chick Embryo;
DNA, Viral;
chemistry;
Molecular Sequence Data;
Phylogeny
- From:
Chinese Journal of Virology
2008;24(5):369-375
- CountryChina
- Language:Chinese
-
Abstract:
Two Avian leukosis viruses of subgroup J (ALV-J) were isolated from layers by inoculating the sample into chicken embryo fibroblast (CEF) cells and by indirect fluorescent assay (IFA) with ALV-J specific monoclonal antibody JE-9. Sequence comparison indicated the gp85 identities were only in the ranges of 83.4%-87.3% compared with five international reference strains and 86.4%-89.6% compared with eight Chinese strains isolated from white meat-type chickens. The gp37 identities were in the range of 91.8-96.4% compared with the five international strains and 93.4-95.9% compared with the eight domestic strains. When compared with the above thirteen strains, two layers isolates were more close to the prototype HPRS-103 and had less deletion in 3'-Ter than those strains. All strains isolated from white meat-type chickens in China had a deletion in the "E" region of 3'-Ter except these two isolates, suggesting these two ALV-J isolates from layers have different evolution origins from other Chinese isolates from white meat-type chickens.
