Rescue of chimeric rabies virus expressing green fluorescent protein.
- Author:
Xiao-Hui LIU
1
;
Jun AI
;
Xiao-Feng GUO
;
Jing-Chen SUN
;
Hong-Ru LIANG
Author Information
1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Line;
Cricetinae;
Green Fluorescent Proteins;
genetics;
Rabies virus;
genetics;
Recombinant Proteins;
genetics
- From:
Chinese Journal of Virology
2008;24(5):401-403
- CountryChina
- Language:Chinese
-
Abstract:
Green fluorescent protein (GFP) gene was inserted into the pseudogene (psi) region of genome of rabies virus rHep-Flury strain, and a recombinant rabies virus carrying GFP, designated as HEP-GFP, was rescued by reverse genetics system. It was demonstrated that green fluorescent protein could be expressed in the chimeric virus after 5 passages in BHK-21 cell line. The research indicated that the pseudogene (psi) region in the genome of rHEP-Flury strain, as an independent functional unit in the process of virus assembly, could independently carry and express exogenous genes.
