Sequence analysis of the nucleocapsid gene and genome promoter region of peste des petits ruminants virus of Chinese origin.
- Author:
Jing-Yue BAO
1
;
Zhi-Liang WANG
;
Lin LI
;
Wen-Ji ZHAO
;
Long-Ci-Ren SUO
;
Jin-Ming LI
;
Ying-Li WANG
;
Xiao-Dong WU
;
Chun-Ju LIU
;
Yu-Tian LIU
;
Xiao-Jing YU
;
Yong-Mei YANG
Author Information
1. National Diagnostic Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao 266032, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
Base Sequence;
China;
Female;
Genome, Viral;
Goat Diseases;
virology;
Goats;
Molecular Sequence Data;
Nucleocapsid Proteins;
chemistry;
genetics;
Peste-des-Petits-Ruminants;
virology;
Peste-des-petits-ruminants virus;
chemistry;
classification;
genetics;
isolation & purification;
Phylogeny;
Promoter Regions, Genetic;
Sequence Alignment;
Sequence Analysis
- From:
Chinese Journal of Virology
2008;24(6):464-471
- CountryChina
- Language:Chinese
-
Abstract:
The N gene and genome promoter nucleotide sequence of a Chinese Peste des petits rumiants virus (PPRV) ("China/Tib/Gej/07-30") was firstly determined. The length of N gene was 1689 nucleotides with a single open reading frame (ORF). The nucleotide and deduced amino acid sequence was compared with the homologous region of other PPRV isolates. The nucleotide sequence of the "China/Tib/Gej/07-30" was 91.7%-97.6% identical to other PPRV isolates, while a homology of 94.9%-98.5% could be observed at the amino acids level. The N gene encoded a protein of 525 amino acids. Several sequence motifs were identified on the basis of conservation in the PPRVs and the morbilliviruses. The genome length of promoter region was 107 nucleotides with 91.8%-98.2% identity to other PPRV isolates. Phylogenetic analysis showed that the "China/Tib/Gej/07-30" belonged to the Asian lineage.
