HPV caused pathological changes in genital system of mice.
- Author:
Wei-wei PAN
1
;
Li-xian CAO
;
Fa-ping YI
;
Ying XU
;
You-quan BU
;
Guo-qi LAI
;
Yong-ping MA
;
Fang-zhou SONG
Author Information
1. Department of Biochemistry and Molecular Biology, Chongqing Medical University. aidspan@163.com
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
Animals;
Blotting, Western;
Cell Line;
Female;
Genital Diseases, Female;
pathology;
virology;
Genitalia, Female;
pathology;
virology;
Humans;
Immunohistochemistry;
Mammary Glands, Animal;
metabolism;
pathology;
Mice;
Mice, Nude;
Oncogene Proteins, Viral;
genetics;
metabolism;
Ovary;
metabolism;
pathology;
Papillomaviridae;
metabolism;
physiology;
Papillomavirus E7 Proteins;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Repressor Proteins;
genetics;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Tumor Suppressor Protein p53;
metabolism;
Uterus;
metabolism;
pathology;
Vagina;
metabolism;
pathology
- From:
Chinese Journal of Virology
2009;25(1):35-40
- CountryChina
- Language:Chinese
-
Abstract:
The recombined adenovirus DNA was transfected into 293 cells for packing and amplification of replication-deficient Ad-CMV-E6/E7, Ad-K14 -E6/E7 virus was purified by CsCl density gradient centrifugation , recombined adenovirus Ad-CMV-E6/E7, Ad-K14 -E6/E7 were used as experimental group, while pAd-CMV and pAdtrack-K14 were used as control group. Four of them were injected through one main vein of nude mice tail respectively. These mice were then treated with 0.05 mg 17beta-estradiol over 12 weeks. Mice were anaesthesiaed with 2.5% Avertint and the vagina, mammary gland, ovaries and uterus were dissected and fixed in 3.75% paraformaldehyde overnight at 4 degrees C. Paraffin-embedded sections, HE staining and identification of P53 and Bcl-2 protein via immunohistochemistry were performed. The expression of E6/E7 was verified by RT-PCR in different tissue of nude mice. HE staining showed evident hyperplasy in cervix-uterus transformation zone of experimental group 2. The expression of mutant P53 and Bcl-2 were higher than control group via immunohistochemical S-P method in uterus stroma-cell. Western blotting also showed that E6 protein was expressed. The expression of E6/E7 was higher than control group by human cytokeratin promoter 14 and hyperlasy changes were detected in epithelial tissue of cervix-uterus transformation zone.
