Complete genomic sequence of a watermelon isolate of cucumber green mottle mosaic virus in northern China.
- Author:
Hong-yun CHEN
1
;
Shi-ming LIN
;
Qing CHEN
;
Wen-jun ZHAO
;
Fu-rong LIAO
;
Hong-jun CHEN
;
Shui-fang ZHU
Author Information
1. Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361012, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
China;
Citrullus;
virology;
Genome, Viral;
genetics;
Molecular Sequence Data;
Tobamovirus;
genetics
- From:
Chinese Journal of Virology
2009;25(1):68-72
- CountryChina
- Language:Chinese
-
Abstract:
The complete genomic sequence of a watermelon isolate of Cucumber green mottle mosaic virus (CGMMV-LN) in Liaoning province was determined and compared with other cucurbit-infecting tobamoviruses. The genomic RNA of CGMMV-LN comprised 6422 nt, and 5'- and 3'- noncoding regions consisted of 59 nt and 175 nt, respectively. The encoded four proteins were two replicase proteins of 186 kD and 129 kD, move protein of 29 kD and coat protein of 17.4 kD. The alignment results of complete nucleotide sequence showed that CGMMV-LN shared identities of 97.6%-99.3% with four other CGMMV isolates, but only shared identities of 61.7%-62.8% with three other tobamoviruses. Homology trees generated from replicase proteins of 186 kD and coat proteins suggested that cucurbit-infecting tobamoviruses could be separated into two subgroups: subgroup I comprising all the isolates of CGMMV and subgroup II comprising Cucumber fruit mottle mosaic virus, Kyuri green mottle mosaic virus and Zucchini green mottle mosaic virus.
