The first report of Kadipiro virus isolation in China.
- Author:
Xiao-hong SUN
1
;
Wei-shan MENG
;
Shi-hong FU
;
Yun FENG
;
You-gang ZHAI
;
Jing-lin WANG
;
Huan-qin WANG
;
Xin-jun LV
;
Guo-dong LIANG
Author Information
1. Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China. dotsunny@hotmail.com
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
Anopheles;
virology;
Cell Line;
China;
Coltivirus;
classification;
genetics;
isolation & purification;
Culex;
virology;
Molecular Sequence Data;
Phylogeny;
Reverse Transcriptase Polymerase Chain Reaction;
Sequence Analysis, DNA
- From:
Chinese Journal of Virology
2009;25(3):173-177
- CountryChina
- Language:Chinese
-
Abstract:
5 strains of virus isolated from Culex tritaeniorhynchus, Anopheles sinensis and Armigeres subalbatus, which caused cytopathic effect in C6/36 cells, had been obtained in the survey of arboviruses in Northwestern Yunnan Province. China. The virus particles displayed 70 nanometers diameter (n=7) with no envelope but spikes on the surfaces. RNA-PAGE of the genomes of the isolates showed 6-5-1 profile. A fragment of the 12th segment sequence was amplified by a pair of specific primers for Kadipiro virus strain JKT-7075 in RT-PCR. The full length of the 12th segment was 758 nucleotides, BLAST analysis revealed the highest identity was 90% to JKT-7075. Phylogenetic analysis demonstrated that the isolates appeared to be Kadipiro viruses (Family Reoviridae). It was the first report of kadipiro virus isolation in China.
