The expression and anti-apoptotic function of HCMV IE2 protein controlled by Tet-On system.
- Author:
Zhi-qiang BAI
1
;
Bin WANG
;
Zhi-jun LIU
;
Ling LI
;
Hai-tao WANG
;
Dong-meng QIAN
;
Zhi-yong YAN
;
Wei ZHAO
;
Xu-xia SONG
;
Shou-yi DING
Author Information
1. Department of Microbiology, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao University Medical College, Qingdao 266071, China. baizhiq@163.com
- Publication Type:Journal Article
- MeSH:
Anti-Bacterial Agents;
pharmacology;
Apoptosis;
drug effects;
genetics;
Doxycycline;
pharmacology;
Gene Expression Regulation;
drug effects;
genetics;
HeLa Cells;
Humans;
Immediate-Early Proteins;
genetics;
metabolism;
Plasmids;
genetics;
Trans-Activators;
genetics;
metabolism;
Tumor Suppressor Protein p53;
metabolism
- From:
Chinese Journal of Virology
2009;25(3):190-195
- CountryChina
- Language:Chinese
-
Abstract:
During the infection of host cells, IE2 protein is one of the first and most abundantly expressed products of HCMV genome, which plays an important role in the controlling of cell cycle and apoptosis. But the correlation between expression level and anti-apoptotic activity of IE2 protein is still not clear. In this study, we successfully established a HCMV IE2 protein expression cell line that was controlled by Tet-On system. The effect of IE2 protein on cell apoptosis and the expression of p53 was detected under different condition of induction. Our results showed that the IE2 protein could inhibit cell apoptosis induced by TNF-alpha. Additionally, the anti-apoptotic activity of IE2 protein seemed to be relevant to its expression level. However, we failed to detect any difference of p53 expression between the IE2 protein expression and non-expression cells. These data indicated that the IE2 protein might inhibit cell apoptosis through regulating different signal pathways.
