Sequencing and analysis of the full coding sequence of Batai virus isolated in China.
- Author:
Feng-Tian WANG
1
;
Zhi LV
;
Jing-Lin WANG
;
Shi-Hong FU
;
Hai-Lin ZHANG
;
Zhi-Yu WANG
;
Guo-Dong LIANG
Author Information
1. School of Public Health, Shandong University, Jinan, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
Base Sequence;
Bunyamwera virus;
genetics;
Cattle;
China;
Clinical Laboratory Techniques;
Cloning, Molecular;
Genome, Viral;
genetics;
Hemorrhagic Fever with Renal Syndrome;
Molecular Sequence Data;
Open Reading Frames;
Polymerase Chain Reaction;
Reassortant Viruses;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Sequence Alignment;
Sequence Analysis, DNA
- From:
Chinese Journal of Virology
2009;25(2):83-87
- CountryChina
- Language:Chinese
-
Abstract:
By RT-PCR and TAIL-PCR, the full coding region of Batai virus isolated in China (YN92-4 strain)was sequenced for the first time. According to the results, the genome of the virus contained three segments S, M and L of 947, 4,371 and 6,860 nucleotides, respectively. The S segment coded a nucleoprotein of 234 amino acids and a nonstructural protein of 102 amino acids, the M and L segments coded a precursor protein of 1 ,435 amino acids and RNA polymerase of 2,239 amino acids, respectively. Compared with the full coding sequence of Batai viruses isolated out of China, the S and M segments of YN92-4 and ON-7/B/01 showed the highest homology in nucleotide and amino acid sequenes with similarity of 97.7% (100%) and 95.7% (98%), respectively. Since there was no full coding sequence information on the L segment in GenBank for the reference, the L segment of YN92-4 was compared with that of Bunyamwera virus and the homology of nucleotide and amino acid was 73.5%and 81.6%, respectively. Phylogenetic analysis showed YN92-4 strain was clustered into one group with the prototype of Batai virus (MM2222). The results suggested that the YN92-4 strain had no occurrance of genetic reassortment (like Ngari virus) and was close to the Batai virus (ON-7/B/01 strain) isolated from cattle serum in Japan.
