Establishment of RT- LAMP for rapid detection of foot-and-mouth disease virus.
- Author:
Jian LI
1
;
Qin CHEN
;
Wei XIONG
;
Xue-En FANG
Author Information
1. Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai, China. lijiancq@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Animals;
DNA Primers;
Foot-and-Mouth Disease;
prevention & control;
virology;
Foot-and-Mouth Disease Virus;
genetics;
isolation & purification;
Genome, Viral;
genetics;
Nucleic Acid Amplification Techniques;
RNA, Viral;
Reverse Transcriptase Polymerase Chain Reaction;
methods;
Sensitivity and Specificity;
Swine Vesicular Disease;
virology
- From:
Chinese Journal of Virology
2009;25(2):137-142
- CountryChina
- Language:Chinese
-
Abstract:
A rapid detection of foot-and-mouth disease virus (FMDV) was established by using reverse transcription loop-mediated isothermal amplification (RT-LAMP) method, meanwhile its specificity and sensitivity were assessed. The results showed that the FMDV RNA could be amplified by incubation at 65degrees C for only 1h using six primers designed based on FMDV polyprotein gene and the amplification products could be detected easily by naked-eye. There is no cross reaction with other virus such as SVDV, SFV and PPV by detecting their RNA samples. The detection limit of this method was found to be 10(-5) dilution of RNA sample which was 100-fold higher than that of PCR and 10-fold higher than real-time PCR.
