Construction of mammalian cell lines continuously expressing JEV structural proteins.
- Author:
Shan LIU
1
;
Ying HUANG
;
Peng YANG
;
Zhi-Wei SUN
;
Wei-Yuan YU
Author Information
1. Lab for Protein Engineering, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
CHO Cells;
Capsid Proteins;
genetics;
metabolism;
Cell Line;
cytology;
virology;
Cricetinae;
Cricetulus;
Encephalitis Virus, Japanese;
genetics;
metabolism;
Gene Expression;
Genetic Vectors;
Viral Structural Proteins;
genetics;
metabolism
- From:
Chinese Journal of Virology
2009;25(4):279-285
- CountryChina
- Language:Chinese
-
Abstract:
Based on the infectious clone of JEV vaccine strain SA14-14-2, prM-E genes and C-prM-E genes were cloned into pCDNA3.1 vector. The recombinant plasmid pCJE-ME was transfected into BHK-21 cells, the expressed proteins were toxic to the cell growth and accelerated the cell death. But when transfected with the plasmid pCJE-CME, the cell lines continuously expressing structural proteins could be selected with G418. And the expression products of pCJE-CME vector could be detected by ELISA, Western Blot and IFA assay. It showed that the JEV capsid protein could enhance the stability of the cell lines expressing the structural proteins. The established cell lines can make the acquirement of the virus-like particles much easier.
