A polymorphism of kynureninase gene in a hypertensive candidate chromosomal region is associated with essential hypertension.
- Author:
Yi ZHANG
1
;
Kui-xing ZHANG
;
Xin HE
;
Wen-tao YUAN
;
Gu-liang WANG
;
Shou-yu MAO
;
Ping-jin GAO
;
Wei HUANG
;
Ding-liang ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Alleles; Asian Continental Ancestry Group; genetics; Exons; Female; Gene Frequency; Humans; Hydrolases; genetics; Hypertension; epidemiology; genetics; Male; Middle Aged; Polymorphism, Single Nucleotide; Promoter Regions, Genetic
- From: Chinese Journal of Cardiology 2005;33(7):588-591
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify single nucleotide polymorphisms (SNP) in the regulatory and coding regions of human kynureninase (KYNU) gene in a hypertensive candidate chromosomal region 2q14-q23 of Han Chinese, and to investigate the relationship between polymorphisms in KYNU and essential hypertension.
METHODSThe SNPs in the promoter region and exons of the KYNU gene were detected by direct DNA sequencing. Genotyping of the nonsynonymous Lys412Glu (A/G) polymorphism was performed by DHPLC technology in 456 hypertensive patients and 430 normal controls.
RESULTSSixteen SNP were identified in the KYNU gene, including 6 in the regulatory region and 2 in the coding region (both of them lead to substitution of amino acid). Significant differences between hypertensive patients and normal controls were observed for the distribution of alleles (chi(2) = 6.693, P = 0.035) and genotypes (chi(2) = 4.188, P = 0.041) of the Lys412Glu polymorphism in all subjects, and for the distribution of alleles in the subgroup of men (chi(2) = 4.424, P = 0.035).
CONCLUSIONThe Lys412Glu polymorphism of the KYNU gene in a hypertensive candidate chromosomal region is associated with essential hypertension in Han Chinese.
