The regulation effect of interleukin-1 on expression of cartilage specificity gene Sox9 mRNA in the human intervertebral discs.
- Author:
Yong ZHAO
1
;
Wen-bo WANG
;
Yu LU
;
Yun GE
;
Zhan-ge YU
;
Fei XUE
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Collagen Type II; genetics; Dose-Response Relationship, Drug; Gene Expression; drug effects; High Mobility Group Proteins; genetics; Humans; Interleukin-1; pharmacology; Intervertebral Disc; cytology; drug effects; metabolism; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; SOX9 Transcription Factor; Time Factors; Transcription Factors; genetics
- From: Chinese Journal of Surgery 2006;44(24):1704-1707
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the regulating effects of interleukin-1 (IL-1) on gene expression of cartilage specificity gene Sox9 and type II collagen mRNA in the human intervertebral discs.
METHODSRT-PCR were used to investigate the effects of IL-1 on gene expression of Sox9 and type II collagen mRNA in intervertebral discs cells cultures of embryo.
RESULTSThe Sox9 and type II collagen mRNA in intervertebral discs were decreased progressively along with the addition concentrations of IL-1 than the controls. And the mRNA of Sox9 and type II collagen also markedly decreased with the time of culture.
CONCLUSIONSIL-1 could cause dose-dependent and time-dependent inhibition effects on Sox9 and type II collagen gene expression in human intervertebral discs.
