Role of c-Jun NH (2)-terminal kinase in insulin resistance after burn.
- Author:
Xin-long CHEN
1
;
Zhao-fan XIA
;
Duo WEI
;
Dao-feng BEN
;
Hong-tai TANG
;
Sheng-de GE
Author Information
- Publication Type:Journal Article
- MeSH: Adaptor Proteins, Signal Transducing; metabolism; Animals; Anisomycin; administration & dosage; Anti-Bacterial Agents; administration & dosage; Blotting, Western; Burns; enzymology; metabolism; physiopathology; Dimethyl Sulfoxide; administration & dosage; Disease Models, Animal; Female; Glucose Clamp Technique; Immunohistochemistry; Injections, Intravenous; Insulin; administration & dosage; Insulin Receptor Substrate Proteins; Insulin Resistance; physiology; JNK Mitogen-Activated Protein Kinases; metabolism; Male; Muscles; metabolism; Phosphorylation; drug effects; Random Allocation; Rats; Rats, Sprague-Dawley; Serine; metabolism; Tyrosine; metabolism
- From: Chinese Journal of Surgery 2007;45(1):62-64
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of c-Jun NH (2)-terminal kinase (JNk) in insulin resistance after burn and its mechanism.
METHODSTwenty-four Sprague-Dawley rats were randomized to control, burn and burn + anisomycin groups. The rats in control group received sham burn trauma, and burn and burn + anisomycin groups received 30% total body surface area (TBSA) full thickness burn injury. Anisomycin (5 mg/kg) together with 250 microl dimethyl sulfoxide (DMSO) was injected to the rats in anisomycin group intravenously, and only 250 microl DMSO in the other two groups. Euglycemic-hyperinsulinemic glucose clamps was performed 2 hours after the injection. The changes of phospho-serine 307, phospho-tyrosine of insulin receptor substrate (IRS)-1 and phospho-JNK in muscle tissues were determined and compared using immunoprecipitation and Western blot analysis or immunohistochemistry in the three groups.
RESULTSThe infusing rates of total 10% glucose (mg x kg(-1) x min(-1)) in control, burn and burn + anisomycin group were 12.3 +/- 0.4, 6.6 +/- 0.3, 6.5 +/- 0.4, respectively. The level of IRS-1 Serine 307 phosphorylation and phospho-JNK in muscle increased significantly, while insulin-induced tyrosine phosphorylation of IRS-1 decreased markedly after burn.
CONCLUSIONSThe activation of JNK elevates the level of IRS-1 phospho-serine 307 and might play a role in insulin resistance after burn in rats.
