The study of photochemical immobilization of urease on polyether sulfone film surface.
- Author:
Sai LI
1
;
Hao FU
;
Xianglin LUO
;
Changxiu WAN
;
Yinping ZHONG
Author Information
1. Chengdu Institute of Organic Chemistry, Chinese Academy of Science, Chengdu 610041.
- Publication Type:Journal Article
- MeSH:
Enzyme Stability;
Enzymes, Immobilized;
metabolism;
radiation effects;
Membranes, Artificial;
Photochemistry;
Polymers;
Sulfones;
Urease;
metabolism;
radiation effects
- From:
Journal of Biomedical Engineering
2002;19(1):13-16
- CountryChina
- Language:Chinese
-
Abstract:
A new method of using photoactivable ester with azido group was described to immobilize urease on polyether sulfone(PES) film surface. The effects of photoactive enzyme concentration, temperature, pH, irradiation time on the activity of immobilized urease were investigated. Reused times and storage stability were also studied. The results showed that the surface concentration of urease immobilized on PES surface was about 0.33 mg/cm2. When the irradiation time was 5 minutes, the relative activity of immobilized urease was the highest and the activity increased with the increase of the concentration of photoactive urease solution. The optimum pH and temperature of immobilized urease were 7 and 50 degrees C respectively. The relative activity of immobilized urease was stable (50%) after 12 times reused at 50 degrees C.
