Study of encapsulation of suicide gene in poly(D,L-lactic-co-glycolic acid) nanoparticles and its characteristics in vitro.
- Author:
Qin HE
1
;
Zhirong ZHANG
;
Ji LIU
;
Min SHU
;
Hua ZHANG
;
Yi LU
;
Fei NIE
;
Peng LUO
Author Information
1. School of Pharmacy, Sichuan University, Chengdu 610041.
- Publication Type:Journal Article
- MeSH:
Biocompatible Materials;
Biodegradation, Environmental;
DNA;
administration & dosage;
Genetic Vectors;
In Vitro Techniques;
Lactic Acid;
Particle Size;
Plasmids;
genetics;
Polyglycolic Acid;
Polymers;
Recombination, Genetic
- From:
Journal of Biomedical Engineering
2002;19(1):30-33
- CountryChina
- Language:Chinese
-
Abstract:
We constructed the AFP promotor, suicide gene and EGFP eukaryotic expression vector recombinant plasmid, and this plasmid DNA was encapsulated by biodegradable, biocompatible polymer PLGA to prepare nanoparticles. The results demonstrate that the mean diameter of DNA-PLGA-NP is 68 nm, the encapsulation ratio reaches to 80%, and the PLGA nanoparticles can protect plasmid DNA from digestion by deoxyribonuclease I (DNaseI) and sonication-induced shearing in vitro.
