Quantitative characterization of adsorbed bovine serum albumin on modified surfaces of poly(ether urethane) materials using ATR-FT-IR spectroscopy.
- Author:
Dongan WANG
1
;
Jian JI
;
Linxian FENG
;
Jiacong SHEN
Author Information
1. Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027.
- Publication Type:Journal Article
- MeSH:
Adsorption;
Animals;
Biocompatible Materials;
Cattle;
Polyethylene Glycols;
Polyurethanes;
Serum Albumin, Bovine;
metabolism;
Spectroscopy, Fourier Transform Infrared;
Stearates
- From:
Journal of Biomedical Engineering
2002;19(1):4-9
- CountryChina
- Language:Chinese
-
Abstract:
Attenuated total reflection (ATR) FT-IR spectroscopy was used to quantitatively characterize the extent of bovine serum albumin (BSA) adsorbed on the surface-coating-modified poly(ether urethane) (PEU) matrix. The two surface modifying additives (SMA) were respectively a tri-block-coupling-polymer of stearyl poly (ethylene oxide)-4,4'-methylene diphenyl diisocyanate-stearyl poly(ethylene oxide), for short MSPEO, and another similar block-coupling polymer with the Cibacron Blue F3G-A endgroups, for short cibaMPEO. The experiments of static BSA adsorption were composed by two parts. One was static isothermal adsorption, and the other was static adsorption kinetics. The quantitative characterization was based on the optical principles of FT-IR, method of experiment and index of the apparatus, by which the enhancement of BSA adsorption on the SMA-modified surfaces was confirmed.
