Expression of integrin alpha 6 in hepatic sinusoidal capillaration.
- Author:
Wenjun XIAO
1
;
Yiping WANG
;
Xiaojing LIU
;
Minghui HUANG
;
Tao GAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, CD; biosynthesis; Carbon Tetrachloride; Immunoblotting; Immunohistochemistry; Integrin alpha6; Laminin; metabolism; Liver; metabolism; pathology; Liver Cirrhosis, Experimental; chemically induced; metabolism; pathology; Male; Rats; Rats, Wistar; Time Factors
- From: Chinese Journal of Hepatology 2002;10(2):90-92
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the expression of integrin alpha 6 in hepatic sinusoidal capillaration.
METHODSThe rat hepatic fibrosis model was established by injection of carbon tetrachloride subcutaneously. Then the expression of laminin and integrin alpha 6 subunit was observed by immunohistochemistry and dot immuno-blotting.
RESULTSWe observed sinusoidal capillaration formed by deposition of laminin along sinusoids in Disse interspace by immunohistochemistry staining. In normal rat the expression of integrin alpha 6 was restricted to portal vascular endothelial cells and bile duct epithelial cell membranes. No expression was observed in sinusoidal endothelial cell membranes. When capillaration integrin alpha 6 was detected in a continuous pattern along the sinusoids, the content of integrin alpha 6 was significantly higher in fibrotic liver tissues than in normal liver tissues as measured by dot immuno-blotting (P<0.05).
CONCLUSIONSDuring fibrogenesis, laminin continuously accumulate in liver tissues and form basement membrane resulting in sinusoidal capillaration, and then induce the expression of integrin alpha 6 on SEC membranes.
