Ursodeoxycholic acid promotes liver regeneration after partial hepatectomy in bile duct obstructive rats.
- Author:
Mingqing XU
1
;
Benli HAN
;
Lan XUE
;
Jianping GONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cholestasis; genetics; physiopathology; surgery; Gene Expression Regulation; drug effects; Hepatectomy; Hepatocyte Growth Factor; genetics; Liver; physiology; surgery; Liver Regeneration; drug effects; Male; Proto-Oncogene Proteins c-met; genetics; RNA, Messenger; drug effects; genetics; metabolism; Rats; Rats, Wistar; Ursodeoxycholic Acid; pharmacology
- From: Chinese Journal of Hepatology 2002;10(2):103-105
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of ursodeoxycholic acid (UDCA) on liver regeneration after 70% partial hepatectomy (PH) in bile duct obstructive (BDO) rats.
METHODSWistar rats were randomly divided into N-PH group in which normal rats were operated with 70% PH, BDO-PH group in which 70% PH were operated after two week's BDO, and BDO-PH UDCA or sterile saline treatment group in which UDCA (15mg kg(-1) d(-1)) or saline was administrated during BDO and after 70% PH. The hepatic pathological changes were observed. BrdU labeling of hepatocytes, the mRNA expression of intrahepatic hepatocyte growth factor (HGF) and its receptor (Met gene) after 70% PH were measured by immunohistochemical analysis and RT-PCR, respectively.
RESULTSImprovements of hepatic function and pathological changes were induced by UDCA administration after BDO. The expression of hepatic HGF/Met mRNA after 70% PH in BDO-PH UDCA treatment group rats was significantly increased compared with N-PH group rats (P<0.05), BrdU peak labelling of hepatocytes (59.39% +/- 10.82%) in BDO-PH UDCA treatment group rats was significantly higher than that (36.22% +/- 8.37%) in BDO-PH group rats (t=4.149, P<0.01) and without significance compared with N-PH group rats (68.64% +/- 11.26%, t=1.451, P >0.05).
CONCLUSIONSUDCA promotes liver regeneration after 70% PH in BDO rats by remission of hepatic pathological changes and elevating hepatic mRNA expression of HGF and Met.
