Expression and identification of specific autoantigens in autoimmune hepatitis.
- Author:
Lieying FAN
1
;
Renqian ZHONG
;
Shaojing ZHANG
;
Hao WANG
;
Jun GU
;
Ye ZHU
;
Huanxing HAN
Author Information
- Publication Type:Journal Article
- MeSH: Autoantigens; genetics; Cytochrome P-450 Enzyme System; genetics; DNA, Complementary; genetics; Electrophoresis, Polyacrylamide Gel; Escherichia coli; genetics; Gene Expression; Hepatitis, Autoimmune; genetics; Humans; Liver; metabolism; Recombinant Fusion Proteins; genetics; metabolism
- From: Chinese Journal of Hepatology 2002;10(2):113-115
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo express and identify soluble liver antigen (SLA) and cytochrome P-450 (CYP 2D6).
METHODSSLA cDNA and CYP 2D6 cDNA were obtained from human liver tissue poly (A)+RNA by RT-PCR. The cDNAs were inserted into fusion expression vector PQE-30 site of BamH I and Hind III. SLA and CYP 2D6 were identified by the SDS-PAGE and Western blot.
RESULTSSDS-PAGE analysis showed that there was a very strong stained band at about 47 kd and 50 kd, respectively. The products could specifically band to anti-SLA or anti-CYP 2D6 autoantibodies.
CONCLUSIONSThe clone and expression of SLA and CYP 2D6 provide useful substances for the diagnosis and research of pathogenesis on autoimmune hepatitis.
