Effect of glycine on the expression of CD(14) gene and protein of hepatic tissue in the course of developing cirrhosis of rats.
- Author:
Jinchun LIU
1
;
Dewu HAN
;
Ruiling XU
;
Yuanchang ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Gene Expression; drug effects; Glycine; pharmacology; Lipopolysaccharide Receptors; biosynthesis; genetics; Liver Cirrhosis; genetics; metabolism; Male; RNA, Messenger; biosynthesis; drug effects; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Hepatology 2002;10(3):181-184
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of glycine on the expression of CD(14) mRNA and protein of hepatic tissue in the course of developing cirrhosis of rats.
METHODSThe cirrhotic model of Wistar rats was established by complex pathogens, who were respectively fed with control diets and control diets adding glycine (1g/d, giving by intragastric infusion) or 5% glycine containing diets at the same time. The rats were sacrificed at 2, 4, and 8 weeks, respectively. Hepatic tissues were collected to measure the expression of CD(14) mRNA and CD(14) protein by the reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot analysis.
RESULTSThe expression of CD(14) mRNA and CD(14) protein in the hepatic tissue of fatty liver and cirrhotic rats fed with diets containing glycine was weaker than their control groups, and the expression of CD(14) mRNA and protein was the weakest in 8 weeks cirrhotic rats fed with the diets.
CONCLUSIONSGlycine can markedly downregulate the expression of CD(14) mRNA and CD(14) protein in hepatic tissues of cirrhotic rats.
