Effect of compound 861 on tissue inhibitor of metalloprotenase 1 gene expression of HSC-T6 cells.
- Author:
Chenghong YIN
1
;
Hong MA
;
Aimin WANG
;
Xuemei MA
;
Jidong JIA
;
Baoen WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Down-Regulation; drug effects; Drugs, Chinese Herbal; pharmacology; Gene Expression; drug effects; RNA, Messenger; biosynthesis; drug effects; Rats; Rats, Sprague-Dawley; Tissue Inhibitor of Metalloproteinase-1; biosynthesis; genetics
- From: Chinese Journal of Hepatology 2002;10(3):197-199
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the in vitro effect of compound 861 (Cpd 861) on tissue inhibitor of metalloprotenase 1 (TIMP1) mRNA levels of HSC-T6 cell.
METHODSHSC-T6 cells were exposed in different concentrations of Cpd 861 (0.25~1.0 mg/ml) for 48 hours. The TIMP1 level was measured by the quantitative reverse-transcription polymerase chain reaction (RT-PCR).
RESULTSThe TIMP1 mRNA levels of HSC-T6 cells at different concentrations of Cpd 861 were lower (2.50 0.71, 0.50 0.01, 0.11 0.03) than those of the normal control (3.78 0.67, P<0.05 or P<0.01).
CONCLUSIONSThe antifibrotic mechanism of Cpd 861 is partly due to its downregulation on TIMP1 mRNA levels of HSC-T6 cells.
