Luteolin inhibits proliferation and collagen synthesis of hepatic stellate cells.
- Author:
Wenxing ZHAO
1
;
Chongli LIANG
;
Zhongmin CHEN
;
Rongqing PANG
;
Bin ZHAO
;
Zhilong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Division; drug effects; Cells, Cultured; Collagen; biosynthesis; drug effects; Expectorants; pharmacology; Flavonoids; pharmacology; Liver; cytology; Luteolin; Protein Synthesis Inhibitors; pharmacology; Rats; Rats, Wistar
- From: Chinese Journal of Hepatology 2002;10(3):204-206
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of luteolin on the proliferation and collagen expression of hepatic stellate cells.
METHODSThe effect of luteolin on proliferation and collagen synthesis of hepatic stellate cells isolated from the liver of Wistar rats were determined by (3)H-TdR and (3)H-Pro, and procollagen gene expression was also detected by DIG-labeled gene probe and in situ hybridization.
RESULTSThe proliferation and collagen synthesis were significantly and dose-dependently inhibited by luteolin when the concentrations reached 10 micromol/L and 20 micromol/L respectively (t=2.542, P<0.05; t=3.650, P<0.01). The type I, III procollagen mRNA expression was decreased by 25 micromol/L luteolin, in which the type I procollagen mRNA was reduced with statistical significance (x(2)=6.850, P<0.01).
CONCLUSIONSLuteolin inhibits the proliferation and collagen expression of hepatic stellate cells in vitro. It may have a preventive or therapeutic role in liver fibrosis.
