HCV RNA assessment by PCR technique for screening post-transfusion HCV infection among blood donors.
- Author:
Wenge XING
1
;
Hongyi XU
;
Rong MA
;
Huaijing ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Blood Donors; Blood Transfusion; Hepacivirus; genetics; isolation & purification; Hepatitis C; prevention & control; Humans; Mass Screening; Polymerase Chain Reaction; RNA, Viral; analysis
- From: Chinese Journal of Hepatology 2002;10(3):211-212
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo survey the application of PCR for screening HCV RNA from blood donations within the window period.
METHODSAccording to a standardized method, 12 blood banks organized by the National Center for Clinical Laboratories collected and prepared about ten thousands specimens. The specimens were tested with two different kits.
RESULTSAmong the 7173 specimens A group, 21 were PCR positive for HCV RNA. The positive rate was 0.29%. There were not positive for HCV RNA among 7477 specimens (B group).
CONCLUSIONSIt is feasible to use the PCR screening for the detection of HCV RNA of blood donations but is unnecessary to standardize the specimen collection and the kit selection.
