Transient expression of fusion protein with a chimeric HBsAg-HSP70 construct in HepG2 cells.
- Author:
Zeng Wei LIANG
1
;
Ying Hua LAN
;
Hong REN
;
Yong Guo LI
;
Da Chuan CAI
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Proteins; Enzyme-Linked Immunosorbent Assay; HSP70 Heat-Shock Proteins; genetics; Hepatitis B Surface Antigens; genetics; Hepatitis B Vaccines; immunology; Humans; Immunohistochemistry; Plasmids; Recombinant Fusion Proteins; genetics; Reverse Transcriptase Polymerase Chain Reaction; Vaccines, DNA; immunology
- From: Chinese Journal of Hepatology 2002;10(4):248-252
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate transient expression of fusion protein with a chimeric HBsAg-HSP70 construct in HepG2 cells.
METHODSEnkaryotic expression plasmids inserted HBsAg gene or chimeric HBsAg-HSP70 gene were prepared and transfected into HepG2 cells by means of cationic liposome. mRNA were detected by RT-PCR and proteins expressed in the cells were detected by immunocytochemistry 48 hours later. HBsAg in cultured supernatants and cell lysates were assayed by ELISA.
RESULTSFusion protein (HBsAg-HSP70) transient expression in HepG2 cells were confirmed by RT-PCR, immunocytochemistry or ELISA, but fusion protein was not assayed in cell cultured supernatants by ELISA.
CONCLUSIONSTransfection of HepG2 cells with a chimeric HBsAg-HSP70 construct leads to express fusion protein, but it does not secrete into cell cultured supernatants.
