Chronobiologic contrast of expression of p53, p16, and cyclin D1 in hepatocarcinoma cells.
- Author:
Wan Xian YU
1
;
Yong Hong ZHANG
;
Yong CAI
;
Xiao Jun ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; chemistry; Cyclin D1; analysis; Cyclin-Dependent Kinase Inhibitor p16; analysis; Female; Humans; Immunohistochemistry; Liver Neoplasms; chemistry; Male; Middle Aged; Tumor Suppressor Protein p53; analysis
- From: Chinese Journal of Hepatology 2002;10(4):253-255
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo contrast the feature of different periods of p53, p16, and cyclin D1 expression in cells of hepatocarcinoma.
METHODSSixty hepatocarcinoma specimens were collected and divided into three periods in one yearly cycle with each period of 20 cases. Hepatocellular carcinoma was documented in all cases by pathomorphological classification. Edmondson-Steinet grading belonged to II~III stages. The expression of gene proteins of p53, p16, and cyclin D1 was determined by immunohistochemical assay (S-P method). The expressive intensity was analysed by the rank test.
RESULTSp16 expression showed significant difference of period (H=10.334, P<0. 05). Arpil-July was the high expressive period.
CONCLUSIONSp16 may take an important role in the chronobiologic mechanism of gene control of hepatocyte canceration and hepatocarcinoma cell growth.
