Epitope mapping of hepatitis C virus non-structure protein 5 from a 7 peptide phage library by using immobilized specific monoclonal antibody.
- Author:
Yan Wei ZHONG
1
;
Jun CHENG
;
Gang WANG
;
Xiao Jun TIAN
;
Xinhua CHEN
;
Yan LIU
;
Li LI
;
Ling Xia ZHANG
;
Ju Mei CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Antibodies, Monoclonal; immunology; Enzyme-Linked Immunosorbent Assay; Epitope Mapping; Hepatitis C; therapy; Peptide Library; Viral Nonstructural Proteins; chemistry; genetics; immunology; Viral Vaccines; immunology
- From: Chinese Journal of Hepatology 2002;10(4):266-268
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen HCV NS5 mimotopes by using monoclonal antibody and phage peptide library.
METHODSBy using HCV NS5 monoclonal antibody as selective molecule, a 7 peptide phage library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.
RESULTSTwelve positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was confirmed as the mimotope of HCV NS5.
CONCLUSIONSHCV mimotope is obtained by phage peptide library screening. The result provides a new approach for HCV therapy and vaccine development.
