Regulative function of extracelluar regulated protein kinases and telomerase in apoptosis of hepatocarcinomatous cell SMMC-7721.
- Author:
Deng Ju LI
1
;
Yao Zhen ZHANG
;
Wen Jing CAO
;
Wei HUANG
;
Wen Li LIU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Carcinoma, Hepatocellular; drug therapy; enzymology; pathology; Etoposide; pharmacology; Harringtonines; pharmacology; Humans; Liver Neoplasms; drug therapy; enzymology; pathology; Mitogen-Activated Protein Kinase 1; antagonists & inhibitors; physiology; Mitogen-Activated Protein Kinase 3; Mitogen-Activated Protein Kinases; antagonists & inhibitors; physiology; Signal Transduction; Telomerase; physiology; Tumor Cells, Cultured; Vincristine; pharmacology
- From: Chinese Journal of Hepatology 2002;10(4):287-288
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the changes of telomerase activity and protein expression of phosphorylated (activated) extracellular regulated protein kinases (ERK1 and ERK2) in the course of inhibiting hepatocarcinomatous cell proliferation and inducing cell apoptosis by three kinds of chemotherapy drugs: Harringtonine (HRT), Vincristine (VCR), and Etoposide (Vp16). To discuss the regulative function to hepatocarcinomatous cell apoptosis and interrelation of telomerase and ERK.
METHODSCytotoxicity assay, flow cytometry analysis, telomerase repeat amplification protocol assay (TRAP), bioluminescence analysis, and western blot were used in this experiment.
RESULTSHRT, VCR, and Vp16 could inhibit cell proliferation (0.28% 0.08%, 0.25% 0.16%, 0.24% 0.11%), induce apoptosis (21.12%, 28.83%, 12.30%), inhibit telomerase activity, and down-regulate the protein expression of phosphorylated ERK.
CONCLUSIONSIt might be through ERK signal transduction pathways that chemotherapy drugs down-regulate telomerase activity and induce apoptosis.
