Effect of RAR-beta transfection on the proliferation and phenotype of rat hepatic stellate cells.
- Author:
Hua LI
1
;
Jin Sheng ZHANG
;
Guang Cun HUANG
;
Nong ZHANG
;
Qi CHEN
;
Xiu Rong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Cell Division; Liver; cytology; Phenotype; Platelet-Derived Growth Factor; pharmacology; Rats; Receptors, Retinoic Acid; physiology; Transfection; Tretinoin; pharmacology
- From: Chinese Journal of Hepatology 2002;10(4):297-300
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of RAR-beta transfection plus treatment with the corresponding ligand ATRA on the proliferation and phenotype of platelet-derived growth factor (PDGF)-activated hepatic stellate cells (HSC).
METHODSPDGF-activated hepatic stellate cells of rats were transfected with eukaryotic expression vector pCMV-script-RAR-beta, which was verified by western blot. The proliferation of transfected HSC was assayed by BrdU incorporation as well as MTT methods. Their phenotype (alpha-SMA and desmin) was observed by immunocytochemistry assay with image analysis and RAR-beta protein expression was detected by western blot.
RESULTSTransfection of RAR-beta gene and treatment with ligand ATRA could increase the expression of RAR-beta protein for at least 144h and inhibit the proliferation and the expression of alpha-SMA and desmin in PDGF-activated HSC. Significant statistical differences were perceived comparing with sham-transfected, only-PDGF treated, non-ligand treated and irrelevant ligand-treated HSC.
CONCLUSIONSTransfected with RAR-beta gene as well as using related ligand ATRA could suppress the proliferation and reverse the activation phenotype of activated HSC.
