Gene expression of two kinds of constitutive nitric oxide synthase in injured spinal cord tissue.
- Author:
Chenglong LIU
1
;
Anmin JIN
;
Chusong ZHOU
;
Shaoxiong MIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Gene Expression; Nitric Oxide Synthase; genetics; Nitric Oxide Synthase Type II; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Spinal Cord Injuries; enzymology; genetics; Up-Regulation
- From: Chinese Journal of Traumatology 2002;5(1):3-6
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the gene expression of two kinds of constitutive nitric oxide synthase (cNOS): neuronal NOS (nNOS) and endothelial NOS (eNOS) in injured spinal cord tissue.
METHODSThirty-six adult Sprague-Dawley rats were divided randomly into six groups: the normal group and the injury groups (2, 6, 12, 24, 48 h after injury, respectively). A compression injury model of the spinal cord wa s ma de and gene expression of nNOS and eNOS were examined by reverse transcription polymerase chain reaction (RT-PCR).
RESULTSThe gene expression of nNOS and eNOS was detected in the normal group and they were up-regulated quickly after injury, reaching the maximum at 6 h. There was no difference between gene expression of nNOS and eNO S in the normal group, but in each injury group the gene expression of eNOS was much higher than that of nNOS.
CONCLUSIONSExpression of constitutive NOS (cNOS) in spinal co rd tissue was up-regulated after injury mainly in the early stage. cNOS as a wh ole offers protection in spinal cord injury, but different cNOS may play different roles.
