Effect of anti-sense oligodeoxynucleotides homeobox B2 on the proliferation and expression of primary human umbilical vein endothelial cells.
- Author:
Xusheng LIU
1
;
Xiaoqi ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Division; Cells, Cultured; Endothelium, Vascular; cytology; Gene Expression; Homeodomain Proteins; genetics; Humans; Oligodeoxyribonucleotides, Antisense; RNA, Messenger; Transcription Factors; genetics; Umbilical Veins; cytology
- From: Chinese Journal of Traumatology 2002;5(1):12-15
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the effect of homeobox B2 (HOXB2) anti sense oligodeoxynucleotides (asodn) on the proliferation and expression of primary human umbilical vein endothelial cells (HUVECs).
METHODSVarious concentrations of HOXB2 asodn modified by thiophosphate transfected the induction of liposome into HUVECs. MTT a nd RT-PCR methods were employed to determine the effect of different conc ent rations of asodn on the endothelial proliferation and the expression level of HOXB2 mRNA.
RESULTSAfter the transfection of HOXB2 asodn, the endothelial proliferation was inhibited in a dose-dependent fashion. Simultaneously, the expression of HOXB2 mRNA decreased significantly.
CONCLUSIONSHOXB2 plays an important role in the proliferation of endothelia.
