Experimental study on the apoptosis of the astrocytes and the oligodendrocytes induced by Ca(2+) reperfusion.
- Author:
Haihan MA
1
;
Yang SHAO
;
Yamin WU
;
Hongyun LI
;
Weihong LIAO
;
Yuan LIU
;
Hengwen YANG
;
Lin ZENG
;
Zaiyun LONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; physiology; Astrocytes; cytology; pathology; physiology; Calcium; physiology; Cells, Cultured; Flow Cytometry; In Situ Nick-End Labeling; Oligodendroglia; cytology; pathology; physiology; Rats; Rats, Wistar
- From: Chinese Journal of Traumatology 2002;5(1):32-35
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the apoptosis rules of the astrocytes and oligodendrocytes induced by Ca(2+) reperfusion.
METHODSThe apoptosis of purified cultured astrocytes and oligodendrocytes induced by Ca(2+) reperfusion and the relationship between the development of the cell apoptosis and post-reperfusion time was observed.
RESULTSBoth the astrocytes and oligodendrocytes were obviously in a time-dependent fashion, and the apoptosis ratios of the oligodendrocytes (39.73%+/-4.16%) were higher than the astrocytes (19.64%+/-4.67%) 24 hours after Ca(2+) reperfusion. The TUNEL positive cells were 13.6+/-1.82 and 21.4+/-1.95 at every visual field of astrocytes and oligodendrocytes respectively 24 hours after Ca(2+) reperfusion.
CONCLUSIONSThe astrocytes and oligodendrocytes are similar wi th the development rules on apoptosis and have different susceptiveness to the situation.
