Identification of a novel HLA allele DRB1*1462 by sequence-based typing.
- Author:
Shuang CUI
1
;
Xiao-Yan SHAN
;
Xiao-Mei HE
;
Na LIU
;
Lin WANG
;
Wei LI
;
Li-Jun WANG
;
Lei NI
;
Dong-Mei WANG
;
Bo-Tao ZHAO
;
Zhi-Yin GONG
;
Zhi-Xin ZHANG
Author Information
1. HLA Laboratory, Beijing Red Cross Blood Center, Beijing 100088, China.
- Publication Type:Journal Article
- MeSH:
Alleles;
Asian Continental Ancestry Group;
genetics;
HLA-DR Antigens;
genetics;
HLA-DRB1 Chains;
Histocompatibility Testing;
Humans;
Molecular Sequence Data;
Sequence Analysis, DNA
- From:
Journal of Experimental Hematology
2009;17(2):493-495
- CountryChina
- Language:Chinese
-
Abstract:
This study was to identify a novel HLA-DRB1 allele in Chinese population by nucleotide sequence ana- lysis. The HLA typing of genes was performed by PCR-SSO and PCR-SSP, the ambiguous novel allele was identified by DNA sequence analysis. The results showed that the sequence of this new allele differed from DRB1*140101 by one nucleotide substitution at position 256 in exon 2 (G- > A), resulting in an amino acid change from Ala to Thr at codon 57. In conclusion, this allele is a novel one, which has been officially given the name DRB1*1462 by the WHO nomenclature committee in January 2006.
