Analysis of variant translocation der ins (17; 15) in patient with APL by G-banding technique and interphase fluorescence in situ hybridization.
- Author:
Tong WANG
1
;
Jing-Ying QIU
;
Chun-Fu YU
;
Xiao-Lan MA
;
Xiao-Peng JIA
;
Yan-Ping WANG
;
Hong-Xing LIU
;
Yue-Hui LIN
;
Chun-Rong TONG
;
Dao-Pei LU
Author Information
1. Laboratory of Cytogenetics, Specific Examination Center, Beijing Daopei Hospital, Beijing, China.
- Publication Type:Case Reports
- MeSH:
Chromosome Banding;
Chromosomes, Human, Pair 15;
Chromosomes, Human, Pair 17;
Humans;
In Situ Hybridization, Fluorescence;
methods;
Interphase;
genetics;
Leukemia, Promyelocytic, Acute;
genetics;
Male;
Translocation, Genetic;
Young Adult
- From:
Journal of Experimental Hematology
2009;17(3):537-540
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the biological characteristics of the variant translocation der ins (17;15) in a patient with acute promyelocytic leukemia (APL), the conventional G-banding technique, interphase fluorescence in situ hybridization (int-FISH), RT-PCR, gene scanning, gene sequence and flow cytometry were performed. The results indicated that the variant translocation der ins (17, 15) observed by G banding technique was a rare type, the int-FISH assay by using dual-color pml/raralpha fusion probes confirmed the cytogenetic findings. The detection results of other molecular methods demonstrated the existence of the whole pml/raralpha fusion gene, while this case had insertion variant translocation. This patient got complete remission by using combined chemotherapy, and survives with continuous complete remission during following up for 1 year. In conclusion, the variant translocation der ins (17; 15) is rare type in APL, its incidence is lower, several signal types in detection of int-FISH were observed and the combination chemotherapy for this patient showed more obvious efficacy.
