Influences of bone marrow mesenchymal stem cells in patients with acute myeloid leukemia and non-leukemia on HL-60 cells -- a comparison study.
- Author:
Wen-Jie YIN
1
;
Ping-Di YANG
;
You-Zhang HUANG
;
Xiao-Peng LI
;
Li-Zhong GONG
Author Information
1. Department of Hematology, PLA Navy General Hospital, Beijing, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
cytology;
Cell Differentiation;
Cell Proliferation;
Coculture Techniques;
HL-60 Cells;
Humans;
Leukemia;
pathology;
Mesenchymal Stromal Cells;
cytology
- From:
Journal of Experimental Hematology
2009;17(3):545-550
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to compare the influences of bone marrow mesenchymal stem cells (BMMSCs) from patients with acute myeloid leukemia (AML), AML patients with complete remission (CR) and non-leukemia patients on HL-60 cells. The HL-60 cells were divided into three groups: group of co-cultivation with BMMSCs of AML patients, group of co-cultivation with BMMSCs of AML patients with CR and group of co-cultivation with BMMSCs of non-leukemia patients. The count of HL-60 cells, the CD11b and survivin expression of HL-60 cells, the cell cycle distribution of the HL-60 cells in 3 groups were compared by flow cytometry, the morphology and differentiation rate of HL-60 cells in 3 groups were observed and compared by microscopy. The results showed that there were no differences in HL-60 cell count at five and seven days, in HL-60 distribution at the G(0)/G(1) phase, in survivin and CD 11b expressions in 3 groups. All cells of 3 groups began to mature, and the differentiation rates in 3 groups were 18.0 +/- 3, 17.0 +/- 1.3 and 19.0 +/- 2.0 respectively, therefore there were no significant differences between the 3 groups (p = 0.23). It is concluded that there is no influence of BMMSCs in 3 groups on the proliferation and differentiation of HL-60 cells.
