Comparison of the solution of ThinPrep(TM) Pap test with the Cervical Sampler(TM) of Hybrid Capture (HC) II for detecting Human papillomavirus (HPV) DNA.
- Author:
So Jin YEO
1
;
Mi Kyeong KANG
;
Kei Hyun NAM
;
Jeong Sig KIM
;
Tae Hee KIM
;
Kwon Hae LEE
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Soonchunhyang University, Bucheon, Korea. khnam@schuh.ac.kr
- Publication Type:Original Article
- Keywords:
Cervical Sampler(TM);
Preserv Cyt(TM) solution
- MeSH:
Cervix Uteri;
DNA*;
Female;
Humans*
- From:Korean Journal of Gynecologic Oncology
2005;16(1):70-76
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Our purpose was to compare the difference and the positivity rate between Preserv Cyt(TM) solution of Thin-Prep(TM) Pap test and Cervical Sampler(TM) of Hybrid Capture II for detecting HPV DNA. METHODS: We collected samples of the cervix by using Preserv Cyt(TM) solution of Thin-Prep(TM) Pap test and a Cervical Sampler(TM) of Hybrid Capture II from ninety one women who screened for cervical carcinoma and precancerous cervical lesions between January 2003 and March 2003. At the same time, we tested the sample using the Preserv Cyt(TM) solution left over with a sample conversion kit. The cut off value for positive test result was above 1 pg/mL. RESULTS: The results of comparing the test using the Preserv Cyt(TM) solution of Thin-Prep(TM) Pap test with the one using Cervical Sampler(TM) showed negative results and 34 patients showed positive results with a significant high kappa coefficient of 0.674. The HPV titer of <1 were considered negative, while the HPV titer of 1 were considered positive. The positive HPV titer results were then subdivided into values of 1-10 and >10. Agreement rates of Preserv Cyt(TM) solution according to the HPV titer of Cervical Sampler(TM) were as follows: 97.7% in values below 1, 96.8% in values above 10, but 17.6% in values 1 to 10. So, the agreement rate was low between Cervical Sampler(TM) and Preserv Cyt(TM) solution in low value of HPV of Cervical Sampler(TM). CONCLUSION: Comparing the test using Preserv Cyt(TM) solution with Cervical Sampler(TM), there was a high correspondence and the Preserv Cyt(TM) solution was facilitated to detection for HPV at a time.
