Influence of As(2)O(3) on proteasome beta(1)-subunit in NB4 cells.
- Author:
Xiao-Hong LÜ
1
;
Ying CHEN
;
Mei ZHANG
;
Peng-Cheng HE
;
Huai-Yu WANG
;
Zeng-Feng NI
;
Rong LU
Author Information
1. Department of Rheumatology, The First Affiliated Hospital, Xi'an Jiaotong University Medical College, Xi'an, Shaanxi Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Arsenicals;
pharmacology;
Cell Differentiation;
drug effects;
Humans;
Leukemia, Promyelocytic, Acute;
metabolism;
Oncogene Proteins, Fusion;
metabolism;
Oxides;
pharmacology;
Proteasome Endopeptidase Complex;
drug effects;
Tumor Cells, Cultured
- From:
Journal of Experimental Hematology
2009;17(3):579-582
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the correlation between effects of arsenic trioxide on NB4 cell differentiation and the change of beta(1)-subunit of 26S proteasome. NB4 cell in 0.5 micromol/L As(2)O(3) was incubated for 24 hours and 48 hours, then total protein was extracted, expressions of subunit beta(1) and PML-RARalpha fusion protein were determined by Western blot. The results indicated that the expression of 26S proteasome beta(1)-subunit increased after incubation with As(2)O(3) for 24 hours, but after culture with As(2)O(3) for 48 hours, the expression of beta-subunit decreased to the baseline. Meanwhile, the expression of PML-RARalpha fusion protein obviously decreased after 24 hours, and kept low level at 48 hours. It is concluded that the expression of 26S proteasome beta(1)-subunit increases after exposure to As(2)O(3). Increment of 26S proteasome beta(1)-subunit may be associated with the degradation of PML-RARalpha fusion protein and plays roles in the differentiation and apoptosis of NB4 cells.
