Protein RAP1GAP in human myelodysplastic syndrome detected by flow cytometry and its clinical relevance.
- Author:
Stella Aprilia IKA
1
;
Xiao-Fei QI
;
Zi-Xing CHEN
Author Information
1. Jiangsu Institute of Hematology, Leukemia Research Unit, First Affiliated Hospital, Suzhou University, Suzhou, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Female;
Flow Cytometry;
GTPase-Activating Proteins;
genetics;
metabolism;
Gene Expression Profiling;
Humans;
Male;
Middle Aged;
Myelodysplastic Syndromes;
genetics;
metabolism
- From:
Journal of Experimental Hematology
2009;17(3):612-617
- CountryChina
- Language:English
-
Abstract:
Previous study on the gene expression profile of human MDS by using microarray discovered that transcription of RAP1GAP was up-regulated, which was confirmed by quantitative RT-PCR in expanding cohort of MDS patients. This study was pourposed to investigate the expression of RAP1GAP in human MDS and its clinical relevance. The expression of RAP1GAP in bone marrow cells of 19 MDS patients was detected by flow cytometry and was compared with that in patients with non-malignant blood diseases and acute leukemias, meanwhile the relevance between expression level of RAP1GAP and hemoglobin, leukocytes, platelets, blasts percentage in bone marrow cells and IPSS score was analyzed. The results indicated that the expression level of RAP1GAp in MDS patients significantly increased as compared with patients with non-malignant blood diseases or AML (8.42 +/- 8.37% vs 2.97 +/- 4.75% or 2.26 +/- 4.24%). Among MDS patients, the expression level of RAP1GAP in MDS-RA was significantly higher than that in MDS-RAEB (11.64 +/- 9.07% vs 4.37 +/- 4.65%). However, no definitive correlation of expression level with above-mentioned clinical parameters was found in detected patients with DMS. In conclusion, the expression of RAP1GAP in MDS patients obviously increases, the relationship between expression level of RAP1GAP and laboratory hematological parameter and IPSS score does not be confirmed. The role played by RAP1GAP expression in the pathogenesis of MDS and its clinical significance during progression of MDS towards AML deserves further studies.
