Expression of Toll-like receptors in human bone marrow mesenchymal stem cells.
- Author:
Xiao-Xia HE
1
;
Hai BAI
;
Guo-Rong YANG
;
Yong-Jie XUE
;
Ya-Nan SU
Author Information
1. Hematologic Disease Center, Lanzhou Military Area Lanzhou General Hosipital, Lanzhou, Gansu Province, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
metabolism;
Cell Differentiation;
Cells, Cultured;
Humans;
Mesenchymal Stromal Cells;
metabolism;
RNA, Messenger;
genetics;
Toll-Like Receptors;
metabolism
- From:
Journal of Experimental Hematology
2009;17(3):695-699
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to explore the characteristics of Toll-like receptor expression in mesenchymal stem cells derived from bone marrow of healthy donor (BM-MSCs). BM-MSCs were isolated from bone marrow of healthy donor by Ficoll method. Expressions of CD34, CD45, HLA-DR, CD44 and CD71 in BM-MSCs were detected by flow cytometry. CD71 in BM-MSCs was assayed by immunocytochemistry. The adipocyte and osteoblast induction of BM-MSCs were detected by alizarin red stain and oil red stain respectively. TLR 1 - 10 mRNA levels in BM-MSCs were evaluated by semiquantitative RT-PCR. The results showed that expressions of CD34, CD45 and HLA-DR in BM-MSC were negative while the expressions of CD44 and CD71 were positive. CD71 in BM-MSCs was positive. After induced by osteoblast and adipocyte inductor, BM-MSCs were positive for alizarin red staining and oil red staining respectively. All of TLR 1 - 10 mRNA were found in BM-MSCs with high expression levels of TLR2, TLR3, TLR4, TLR7, TLR8, TLR9 and low expression levels of TLR1, TLR5, TLR6, TLR10. In conclusion, different levels of TLR 1 - 10 mRNA were expressed in BM-MSCs of healthy donor.
