Dynamic analysis of expression of VEGF and its receptor-2 in mouse model with acute myeloid leukemia.
- Author:
Ri-Ling CHEN
1
;
Ming-Zhen CHEN
;
Qian-Ying YIE
;
Chuan TIAN
;
Huan WANG
Author Information
1. Department of Pediatrics, Hospital Affiliated to Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China. crl319@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow;
pathology;
HL-60 Cells;
Humans;
Mice;
Mice, SCID;
Neoplasm Transplantation;
Vascular Endothelial Growth Factor A;
metabolism;
Vascular Endothelial Growth Factor Receptor-2;
metabolism
- From:
Journal of Experimental Hematology
2009;17(4):852-856
- CountryChina
- Language:Chinese
-
Abstract:
The objective of study was to explore the role of vascular endothelial growth factor (VEGF) and its receptor-2 in pathogenesis of acute myeloid leukemia. The acute myeloid leukemia model was established on 20 mice with severe combined immunodeficiency (SCID) transplanted by HL-60 cells. The mice were divided into the normal control and test group randomly. The expression of VEGF was detected by enzyme linked immunosorbent assay (ELISA). The expression of VEGFR-2 mRNA was detected by RT-PCR. The results showed that the establishment of acute myeloid leukemia model was succeeded on all SCID mice by HL-60 cell transplantation. The expressions of VEGF and VEGFR-2 mRNAs could be determined on all mice. As compared with the normal control group, the expressions of VEGF and VEGFR-2 mRNAs in the test group significantly increased, but gradually increased during the course of disease. It is concluded that the abnormal expressions of VEGF and VEGFR-2 exist in mice with acute myeloid leukemia, which may be involved in the pathogenesis of AML.
