Coix lachryma jobi L varma-yuan induces apoptosis of jurkat cell line in acute T lymphoblast leukemia and its mechanism.
- Author:
Gen-Hong YAO
1
;
Guo-Dong ZHANG
;
Jian-Feng LUAN
;
Dong YE
;
Jin-Mei YAN
;
Pei-Yuan ZHU
;
Qian-Hong LEI
Author Information
1. Department of Blood Transfusion, Nanjing General Hospital, Nanjing Military Area of PLA, School of Medicine, Nanjing University, Nanjing 210002, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Proliferation;
drug effects;
Coix;
chemistry;
Humans;
Jurkat Cells;
Membrane Potential, Mitochondrial;
Plant Oils;
pharmacology
- From:
Journal of Experimental Hematology
2009;17(4):879-882
- CountryChina
- Language:Chinese
-
Abstract:
The aim of the present study was to investigate the anti-proliferation and pro-apoptosis effect of Coix lachrymajobi L varma-yuan on acute T lymphoblast leukemia cell line Jurkat cells and its mechanism. Jurkat cells were treated with Coix lachrymajobi L varma-yuan of various concentrations (0, 0.4, 0.8, 1.6 mg/ml) for 24h. The inhibitory ratio was measured by Cell Counting Kit-8. The effects of Coix lachrymajobi L varma-yuan on apoptosis of Jurkat cells were determined by Hoechst 33258, PI and Annexin V-FITC/PI double staining. The mitochondrial membrane potential was analyzed by JC-1 staining. The results demonstrated that Coix lachrymajobi L varma-yuan inhibited the proliferation of Jurkat cells, and induced chromatin condensation and fragmentation (characteristic of apoptosis) and loss of mitochondrial membrane potential. In conclusion, Coix lachrymajobi L varma-yuan can inhibit the cell proliferation and induce the apoptosis of Jurkat cells. These effects relate to loss of mitochondrial membrane potential. These results suggest that Coix lachrymajobi L varma-yuan may be of value in treating lymphoma.
