Effect of demethylating treatment on cytotoxicity of NK-92MI cells.
- Author:
Xiao-Ning GAO
1
;
Li-Li WANG
;
Ji LIN
;
Li YU
Author Information
1. Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Cytotoxicity, Immunologic;
immunology;
DNA Methylation;
Flow Cytometry;
Gene Expression Regulation;
Humans;
K562 Cells;
Killer Cells, Natural;
immunology;
metabolism;
Receptors, KIR2DL1;
metabolism;
Receptors, KIR2DL3;
metabolism;
Receptors, KIR3DL1;
metabolism;
Receptors, KIR3DL2;
metabolism
- From:
Journal of Experimental Hematology
2009;17(4):924-928
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the effect of demethylating treatment on the expression of inhibitory KIR and the cytolytic activity of NK-92MI cells, and to study the possible relationship between the demethylation of inhibitory kir gene and the function of NK cells. NK-92MI cells were treated with 5-azacytidine to induce DNA demethylation. The expression of KIR3DL1, KIR2DL2/KIR2DL3, KIR2DL1 and the viability of NK-92MI cells were detected by flow cytometry. The KIR3DL1 positive and the KIR3DL1 negative NK-92MI cells were also sorted by flow cytometry. The cytotoxicity of NK-92MI against K562 cells was detected by the LDH release assay. The results demonstrated that the expressions of KIR3DL1, KIR2DL2/KIR2DL3 and KIR2DL1 in NK-92MI cells all increased after treating with 1.0, 2.5 and 5 micromol/L of 5-azacytidine for 72 hours. And the cytotoxicity of NK-92MI against K562 cells decreased. In these dose range, 5-azacytidine did not influence the viability of NK-92MI cells. Additionally, the cytotoxicity of KIR3DL1 positive NK-92MI cells was lower than that of the KIR3DL1 negative cells. It is concluded that the demethylating treatment suppresses the cytotoxicity of NK-92MI cells through increasing the expression of inhibitory KIR.
