Lethal effect of cytotoxic lymphocytes against U266 cells induced by DCs modified with GM-CSF gene and pulsed with tumour antigen.
- Author:
Chun-Tuan LI
1
;
Xiong-Peng ZHU
;
Wen-Qian XU
;
Hui-Fang XIAO
;
Zhi-Gao DONG
Author Information
1. Department of Hematology, Quanzhou First Hospital, Fujian Medical University, Quanzhou 362000, Fujian Province, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
Antigens, Neoplasm;
genetics;
immunology;
Cancer Vaccines;
immunology;
Cell Line, Tumor;
Cytotoxicity, Immunologic;
Dendritic Cells;
immunology;
Granulocyte-Macrophage Colony-Stimulating Factor;
genetics;
immunology;
Humans;
Multiple Myeloma;
Recombinant Proteins;
T-Lymphocytes, Cytotoxic;
immunology;
Transfection
- From:
Journal of Experimental Hematology
2009;17(4):929-932
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to investigate the lethal effect of cytotoxic lymphocytes against U266 cells induced by DCs pulsed with multiple myeloma (MM) U266 lysate and transfected with GM-CSF recombinant adenovirus. The cytotoxic lymphocytes against U266 cells were induced by culturing with DCs, which pulsed with MM U266 antigens and transfected with GM-CSF recombinant adenovirus. The effect of cytotoxic lymphocytes against U266 cells were measured by LDH release detection. Experiments were divided into 3 groups: N-DC group as control in which DCs were normal; U-DC group in which DCs were pulsed by U266 soluble antigen, and G-U-DC group in which DCs were stimulated by U266 soluble antigen and GM-CSF transfected with Ad-CMV. The results showed that there was significant difference on killing rate against U266 cells between 3 groups (F = 10.939, p < 0.05). The killing rate of G-U-DC group was the highest (p < 0.001), and killing rate of U-DC group was higher than that of N-DC group (p < 0.001). It is concluded that the cytotoxic lymphocytes against U266 cells can be induced by DCs pulsed with U266 lysate, and the lethal effect of CTLs can be enhanced when DCs transfected by recombinant adenovirus with exogenous gene GM-CSF.
