Study of loss of heterozygosity in oligodendroglial tumors by real-time quantitative polymerase chain reaction-based microsatellite analysis.
- Author:
Shu-kun ZHANG
1
;
De-hong LU
;
Yue-shan PIAO
;
Yan-ning CAI
;
Qing-zhong XU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Brain Neoplasms; genetics; Chromosomes, Human, Pair 1; Chromosomes, Human, Pair 19; Female; Humans; Loss of Heterozygosity; Male; Microsatellite Repeats; Middle Aged; Oligodendroglioma; genetics; Polymerase Chain Reaction; methods
- From: Chinese Journal of Pathology 2006;35(12):731-734
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the loss of heterozygosity (LOH) at chromosomes 1p or 19q in oligodendroglial tumors.
METHODSTwenty-eight cases of oligodendroglial tumors were enrolled into the study. Real-time quantitative polymerase chain reaction-based microsatellite analysis was performed on paraffin-embedded tumor tissues in order to study the status of chromosomes 1p and 19q.
RESULTSAmong the 28 cases of oligodendroglial tumors, 24 cases (85.7%) showed 1p LOH, while 18 cases (64.3%) showed 19q LOH and 17 cases (60.7%) showed LOH of both 1p and 19q. LOH at 1p or 19q was present in 25 (89.3%) of the 28 cases.
CONCLUSIONSReal-time quantitative polymerase chain reaction-based microsatellite analysis is a rapid and specific way in detecting LOH in paraffin-embedded tumor tissues. LOH at 1p or 19q is present in majority of the oligodendroglial tumors studied.