Expression of origin recognition complex 1 gene in DNA replication of vascularsmooth muscle cells.
- Author:
Ming-Hong JIANG
1
;
Mao-Qin SHU
;
Yue-Long QIN
;
Qian WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta, Thoracic; cytology; Blotting, Western; Cell Cycle; Cell Proliferation; Cells, Cultured; DNA; genetics; DNA Replication; Gene Expression Regulation, Neoplastic; Male; Muscle, Smooth, Vascular; cytology; Myocytes, Smooth Muscle; metabolism; Origin Recognition Complex; biosynthesis; genetics; physiology; RNA, Messenger; metabolism; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Pathology 2007;36(2):122-125
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the expression of origin recognition complex 1 (ORC1) during the DNA replication of vascular muscle cells (VSMC).
METHODSVSMC of thoracic aorta in rats were obtained by the adherence method of tissue culture. The cell synchrony was obtained by the method of double-thymidine block, colchicine treatment and serum starvation. The expression of ORC1 mRNA at different cell cycles of VSMC was determined by RT-PCR and the protein expression of ORC1 was analyzed by Western blot.
RESULTSCultured VSMC were identified by light microscope and immunocytochemistry. Significant expression of ORC1 mRNA and protein in a quiescent stage of VSMC were not observed. Upon synchronization, the expression of ORC1 mRNA was significantly higher at G(1)/S phase of VSMC than that at S and G(2)/M phases. The expression of ORC1 protein followed same changes as the ORC1 mRNA expression at different stages of cell cycles.
CONCLUSIONORC1 may be an important regulatory factor at the initiation of proliferative process of VSMC.
