Kaempferol-induced apoptosis of human esophageal squamous carcinoma Eca-109 cells and the mechanism.
- Author:
Rui-jun LI
1
;
Jia-zhuan MEI
;
Gui-ju LIU
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; pharmacology; Apoptosis; drug effects; Carcinoma, Squamous Cell; pathology; Caspase 3; metabolism; Caspase 9; metabolism; Cell Line, Tumor; Cell Proliferation; drug effects; Esophageal Neoplasms; pathology; Humans; Kaempferols; pharmacology; Mitochondria; metabolism; Proto-Oncogene Proteins c-bcl-2; metabolism; bcl-2-Associated X Protein; metabolism
- From: Journal of Southern Medical University 2011;31(8):1440-1442
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the growth-inhibiting and pro-apoptotic effect of kaempferol in human esophageal squamous carcinoma Eca-109 cells and explore the mechanism.
METHODSThe effect of kaempferol on Eca-109 cell proliferation in vitro was measured by MTT assay. TUNEL staining was used to detect the cell apoptosis following kaempferol treatment. The changes in Bax and Bcl-2 mRNA expressions in response to kaempferol treatment were determined by RT-PCR, and the caspase-3 and caspase-9 activities were evaluated using colorimetric assay.
RESULTSKaempferol significantly inhibited Eca-109 cell proliferation (P<0.05) in a concentration-dependent manner and induced obvious cell apoptosis. RT-PCR showed that after kaempferol treatment caused up-regulated Bax and down-regulated Bcl-2 mRNA expression. The colorimetric assay revealed significantly increased caspase-3 and caspase-9 activities in Eca-109 cells following kaempferol treatment (P<0.01).
CONCLUSIONKaempferol can induce apoptosis of Eca-109 cells via a mitochondria-dependent pathway.
