Induced differentiation of endometrial stromal stem cells into osteoblast and chondroblasts.

Xin-yuan Yang; Wei Wang; Wei Chen; Xu LI

Return

Induced differentiation of endometrial stromal stem cells into osteoblast and chondroblasts.

Author: Xin-yuan YANG ¹ ; Wei WANG ; Wei CHEN ; Xu LI
Author Information

1. Department of Gynecology and Obstetrics, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China. xinyuan@stu.xjtu.edu.cn
Publication Type:Journal Article
MeSH: Adult; Cell Differentiation; Cells, Cultured; Chondrocytes; cytology; Endometrium; cytology; Female; Humans; Middle Aged; Osteoblasts; cytology; Stromal Cells; cytology
From: Journal of Southern Medical University 2011;31(9):1488-1492
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the capacity of human endometrial stromal stem cells for differentiation into osteoblasts and chondroblasts and their potential as seeding cells in bone tissue engineering.
METHODSHuman endometrial stromal stem cells were obtained from hysterectomy tissues from 15 women during normal menstrual cycles and induced to differentiate into osteoblasts and chondroblasts. The differentiated cells were examined with cytochemistry.
RESULTSA population of endometrial stromal stem cells was successfully isolated from human endometrial tissue and showed stable proliferation in vitro. After treatment with osteoblast and chondroblast revulsant, the endometrial stromal stem cells differentiated towards osteoblasts were verified by positive staining with alizarin red and towards chondroblasts by positive staining with Alcian blue.
CONCLUSIONEndometrial stromal stem cells obtained from human endometrial tissue with multilineage potential can differentiate into osteoblasts and chondroblasts in vitro, and may serve as candidate autogenous seeding cells for bone tissue engineering.