Development of a time-resolved fluoroimmunoassay for detecting S1 subunit of pertussis toxin and its application.
- Author:
Qiang MA
1
;
Guan-feng LIN
;
Li-ping ZOU
;
Ming LI
;
Ying-song WU
Author Information
- Publication Type:Journal Article
- MeSH: Cross Reactions; Fluoroimmunoassay; methods; Pertussis Toxin; analysis; Pertussis Vaccine; chemistry; standards; Quality Control; Sensitivity and Specificity
- From: Journal of Southern Medical University 2011;31(9):1509-1512
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a time-resolved fluoroimmunoassay (TRFIA) for detection of pertussis toxin (PT) S1 subunit for quality control of human PT vaccine.
METHODSA double antibody sandwich one-step method was used to establish the TRFIA for detecting PT S1 subunit in the vaccine.
RESULTSThe sensitivity of c peptide analysis reached 2.5 ng/ml without cross-reactions with other antigens. This assay could be used in detecting S1 subunit in the vaccine.
CONCLUSIONThe TRFIA for detecting PT S1 subunit is simple, sensitive and rapid for quality control of the PT vaccine.
