Metabolic footprint in conditioned culture medium of placental explants: a comparison between early-onset and late-onset severe preeclampsia.
- Author:
Da-yan LIU
1
;
Shi-ling CHEN
;
Chen-hong WANG
;
Xue-mei LUO
;
Fang-fang HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Chorionic Villi; metabolism; Culture Media, Conditioned; chemistry; Female; Humans; In Vitro Techniques; Kynurenine; metabolism; Ornithine; metabolism; Placenta; metabolism; Pre-Eclampsia; metabolism; Pregnancy; Tryptophan; metabolism
- From: Journal of Southern Medical University 2011;31(9):1547-1550
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the differences of metabolic footprint in the conditioned culture medium of placental explants between early-onset and late-onset severe preeclampsia.
METHODSIn 13 cases of early-onset severe preeclampsia and 14 cases of late-onset severe preeclampsia, the placentas were sampled at the surface of the maternal placenta. High performance liquid chromatography-mass spectrometry (HPLC-MS) was used to determine the differences in the metabolites in the conditioned culture medium of the placental villous explants cultured in 6% atmospheric O(2) for 96 h. Standard samples were used to establish the tryptophan and kynurenine chromatography library by HPLC-MS to analyze the concentration of tryptophan and kynurenine in the conditioned culture medium.
RESULTSThirty-six metabolites showed statistically significant differences between early-onset and late-onset severe preeclampsia (P<0.05). The concentration of kynurenine was significantly higher in early-onset severe preeclampsia than in late-onset severe preeclampsia (P<0.05).
CONCLUSIONEarly-onset and late-onset severe preeclampsia may have different pathogeneses. By detecting the concentration of metabolites, metabolomic strategies provide a new means for predicting the onset time of severe preeclampsia.